Journal
/
암 연구학
/
Development of a Human Preclinical Model of Osteoclastogenesis from Peripheral Blood Monocytes Co-cultured with Breast Cancer Cell Lines
JoVE 신문
암 연구학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 암 연구학
Development of a Human Preclinical Model of Osteoclastogenesis from Peripheral Blood Monocytes Co-cultured with Breast Cancer Cell Lines

Development of a Human Preclinical Model of Osteoclastogenesis from Peripheral Blood Monocytes Co-cultured with Breast Cancer Cell Lines

DOI:
10.3791/56311-v

•

06:00 min

•

September 13, 2017

•

, , , , , , , ,
1Osteoncology and Rare Tumors Center, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST),IRCCS

Chapters

  • 00:05Title
  • 01:17Osteoclast Differentiation and Cancer Cell Co-culture
  • 04:06Results: Representative Effects of Everolimus on Osteoclastogenesis Treatment
  • 04:51Conclusion

Summary

자동 번역

자동 번역

This protocol describes development of an in vitro human preclinical model of osteoclastogenesis from peripheral blood monocytes cultured with breast cancer cell lines to mimic the cancer cell-osteoclast interaction. The model could be used to further our understanding of bone metastasis formation and improve therapeutic options.

Tags

OsteoclastogenesisPeripheral Blood MonocytesBreast Cancer Cell LinesBone MetastasisPreclinical ModelPBMCMCSFTRAP Staining

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/56311?article_type=v

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies
check_url/kr/56311?article_type=v

Surgical Procedures and Methodology for a Preclinical Murine Model of De Novo Mammary Cancer Metastasis
check_url/kr/56311?article_type=v

A Portal Vein Injection Model to Study Liver Metastasis of Breast Cancer
check_url/kr/56311?article_type=v

A Syngeneic Mouse Model of Metastatic Renal Cell Carcinoma for Quantitative and Longitudinal Assessment of Preclinical Therapies
check_url/kr/56311?article_type=v

A Mouse Model of Fatigue Induced by Peripheral Irradiation
check_url/kr/56311?article_type=v

Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish
check_url/kr/56311?article_type=v

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment
check_url/kr/56311?article_type=v

Establishment and Characterization of Three Afatinib-resistant Lung Adenocarcinoma PC-9 Cell Lines Developed with Increasing Doses of Afatinib
check_url/kr/56311?article_type=v

Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia
check_url/kr/56311?article_type=v

A Human Peripheral Blood Mononuclear Cell (PBMC) Engrafted Humanized Xenograft Model for Translational Immuno-oncology (I-O) Research

Read Article