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Hydrodynamisk nyrebækken injektion for ikke-virale udtryk af proteiner i nyrerne

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Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney

Hydrodynamisk nyrebækken injektion for ikke-virale udtryk af proteiner i nyrerne

DOI:

10.3791/56324-v

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08:26 min

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January 08, 2018

•

Lauren E. Woodard^2,3, Richard C. Welch, Felisha M. Williams, Wentian Luo, Jizhong Cheng, Matthew H. Wilson^2,3

¹Department of Veterans Affairs,Tennessee Valley Healthcare System, ²Departments of Medicine and Pharmacology,Vanderbilt University Medical Center, ³Department of Medicine,Baylor University College of Medicine

Chapters

00:05Title
00:52Preparation for Surgery
01:39Injection Surgery
04:03Injection Efficiency and Transgene Effects Assessment
06:06Results: Hydrodynamic Renal Pelvis Injection Results in Kidney-specific Gene Expression
07:25Conclusion

Summary

자동 번역

Denne protokol beskriver en metode til at injicere plasmid DNA i musenyre via nyrebækken at producere transgen udtryk specielt i nyrerne.

Tags

Hydrodynamic Renal Pelvis Injection Protein Expression Plasmid DNA Kidney Nephrology Visual Demonstration Surgical Procedure Mouse Anesthesia Incision Kidney Exposure Plasmid DNA Injection

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