MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening

Mavis Mensah; Cristina Borzi; Carla Verri; Paola Suatoni; Davide Conte; Ugo Pastorino; Fortunato Orazio; Gabriella Sozzi; Mattia Boeri

doi:10.3791/56326

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MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening

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MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening

DOI:

10.3791/56326-v

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08:14 min

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October 26, 2017

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Mavis Mensah*¹, Cristina Borzi*¹, Carla Verri, Paola Suatoni, Davide Conte, Ugo Pastorino, Fortunato Orazio, Gabriella Sozzi, Mattia Boeri

¹Department of Experimental Oncology and Molecular Medicine,Fondazione IRCCS Istituto Nazionale dei Tumori, ²Unit of Thoracic Surgery,Fondazione IRCCS Istituto Nazionale dei Tumori

Chapters

00:05Title
01:12Plasma Sample Collection and RNA Extraction
03:20Taq-based Reverse Transcription and Pre-amplification
04:34RT-qPCR Reaction
05:46Results: Quality Control of Plasma Samples and RT-qPCR Performance Evaluation
07:15Conclusion

Summary

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Here, we present the detailed protocol adopted in the BioMILD screening trial to perform the circulating microRNA signature classifier test for early lung cancer detection.

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening

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Summary

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