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Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel
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암 연구학
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Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel

Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel

DOI:
10.3791/56327-v

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06:44 min

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September 08, 2017

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, , , , ,
1Department of Pathology, Tisch Cancer Institute,Icahn School of Medicine at Mount Sinai, 2Department of Oncological Sciences, Tisch Cancer Institute,Icahn School of Medicine at Mount Sinai

Chapters

  • 00:05Title
  • 00:52Generation of Docetaxel-resistant Prostate Cancer Cells
  • 03:33Functional Characterization of Acquired Docetaxel Resistance by Colony Formation Assay
  • 05:10Results: Generation of Docetaxel-resistant Prostate Cancer Cell Model Systems and Functional Characterization of Docetaxel Resistance
  • 06:14Conclusion

Summary

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자동 번역

Resistance to cancer therapies contributes to disease progression and death. Determining the mechanistic underpinnings of resistance is crucial for improving therapeutic response. This manuscript details the protocol to generate taxane-resistant cell models of prostate cancer (PC) to help dissecting the pathways involved in progression to Docetaxel resistance in PC patients.

Tags

Docetaxel-resistant Prostate Cancer Cell ModelsCancer Cell ResistanceAnti-mitotic Therapy ResistanceDrug Response ModelsCell Line DevelopmentDU145 Cells22Rv1 CellsDose EscalationCell CultureTrypsinizationCell PassagingPooled Resistant Cells

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