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荧光显微镜评价辐射诱发克隆细胞死亡模式的一步协议
JoVE 신문
암 연구학
This content is Free Access.
JoVE 신문 암 연구학
One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy
DOI:

06:47 min

October 23, 2017

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Chapters

  • 00:05Title
  • 01:31Cell Culture and Irradiation
  • 02:53Fixation and DAPI Staining
  • 04:44Results: Nuclear Morpholgy in Different Forms of Cell Death
  • 05:51Conclusion

Summary

자동 번역

电离辐射 (ir) 诱导的克隆细胞死亡的研究对于了解 ir 对恶性肿瘤和正常组织的影响是非常重要的。在这里, 我们描述了基于 4 ', 6-diamidino-2-吲二盐酸盐 (DAPI)-荧光显微镜可视化染色核的形态学的红外诱导克隆细胞死亡主要模式的 one-step 测定。

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