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改善斑马鱼幼虫实验操作的长期活体成像装置
JoVE 신문
생체공학
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JoVE 신문 생체공학
Long-term Live Imaging Device for Improved Experimental Manipulation of Zebrafish Larvae
DOI:

14:05 min

October 27, 2017

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Chapters

  • 00:05Title
  • 00:57Design and 3D Printing of Molds
  • 02:57PDMS Fabrication of zWEDGI Device
  • 04:43Plasma-bonding zWEDGI to Glass Dish
  • 07:19Channel Preparation and Loading Larvae
  • 09:52Wounding and Imaging Larvae and End of Experiment
  • 11:14Results: Post Wound Live Imaging and 3D Reconstruction of Zebrafish Larvae Tail Fin
  • 12:36Conclusion

Summary

자동 번역

这份手稿描述了 zWEDGI (斑马鱼伤人和诱捕装置的增长和成像), 这是一个条块分割的装置, 旨在定位和抑制斑马鱼幼虫。该设计允许尾部横断面和长期收集高分辨率的荧光显微图像的伤口愈合和再生。

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