Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

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Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

DOI:

10.3791/56342-v

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11:11 min

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August 24, 2017

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Xiaoxing Lv, Meiru Zhao, Yuting Yi, Lucheng Zhang, Yanfang Guan, Tao Liu, Ling Yang, Rongrong Chen, Jianhui Ma, Xin Yi

¹Geneplus-Beijing Institute

Chapters

00:05Title
01:04DNA Extraction from Peripheral Blood for cfDNA and Genomics DNA (gDNA)
03:02Library Preparation
06:47Targeted DNA Capture
09:10Results: Next Generation Sequencing to Detect Rare Mutations in ctDNA
10:12Conclusion

Summary

자동 번역

This manuscript describes a technique for detecting mutations of low frequency in ctDNA, ER-Seq. This method is differentiated by its unique use of two-directional error correction, a special background filter, and efficient molecular acquirement.

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

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Summary

자동 번역

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