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3D 基质中哺乳动物细胞分裂的定量共焦反射显微镜观察
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JoVE 신문 생체공학
Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy
DOI:

10:22 min

November 29, 2017

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Chapters

  • 00:05Title
  • 00:32Generation of MDA-MB-231 Cells Stably Expressing Histone H2B-mCherry Using Lentiviral Vectors
  • 04:00Synchronization of Cells Stably Expressing H2B-mCherry
  • 06:02Incorporation of the Synchronized Cells into Collagen I Matrices
  • 07:18Live Cell Imaging and Matrix Deformation During Cell Division in 3D Collagen Matrices
  • 08:46Results: Insights from Imaging Mammalian Cell Division in 3D Collagen Matrices
  • 09:45Conclusion

Summary

자동 번역

该协议有效地研究了3D 胶原基质中的哺乳动物细胞分裂, 结合细胞分裂的同步, 用活细胞成像技术监测3D 矩阵中的分裂事件, 时间分辨共聚焦反射显微镜和定量成像分析。

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