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1Department of Chemical and Biomolecular Engineering,Johns Hopkins University, 2Johns Hopkins Physical Sciences – Oncology Center,Johns Hopkins University, 3Department of Biomedical Engineering,Johns Hopkins University, 4Departments of Oncology and Pathology and Sidney Kimmel Comprehensive Cancer Center,Johns Hopkins University School of Medicine
Chapters
- 00:05Title
- 00:32Generation of MDA-MB-231 Cells Stably Expressing Histone H2B-mCherry Using Lentiviral Vectors
- 04:00Synchronization of Cells Stably Expressing H2B-mCherry
- 06:02Incorporation of the Synchronized Cells into Collagen I Matrices
- 07:18Live Cell Imaging and Matrix Deformation During Cell Division in 3D Collagen Matrices
- 08:46Results: Insights from Imaging Mammalian Cell Division in 3D Collagen Matrices
- 09:45Conclusion
Ce protocole a efficacement étudie la division cellulaire chez les mammifères dans les matrices de collagène 3D en intégrant la synchronisation de la division cellulaire, la surveillance des manifestations de la division dans des matrices 3D à l’aide de la technique d’imagerie de cellules vivantes, microscopie de réflexion confocal résolution temporelle et analyse d’imagerie quantitative.
