Journal
/
면역학 및 감염병학
/
Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

DOI:
10.3791/56373-v

•

09:50 min

•

September 29, 2017

•

, , , , ,
1Dept. of Ecosystem and Public Health, Calgary Prion Research Units, Faculty of Veterinary Medicine,University of Calgary, 2Dept. of Molecular Biology,University of Wyoming, 3Canadian Food Inspection Agency,Lethbridge Laboratories

Chapters

  • 00:05Title
  • 00:42Preparation of Cervid Feces Etracts
  • 01:48Concentration of PrP
  • 03:33Real-time Quaking-induced Conversion Assay
  • 07:28Results: Detection of CWD Prions in Fecal Material
  • 08:36Conclusion

Summary

자동 번역

자동 번역

Here, we present a protocol to describe a simple, fast and efficient prion amplification technique, the real-time quaking-induced conversion (RT-QuIC) method.

Tags

CWD PrionsFecal MaterialReal-time Quaking-induced Conversion AssayCervidChronic Wasting DiseaseSensitive DetectionHigh ThroughputSodium Phosphotungstic Acid PrecipitationRT-QuIC AssayThioflavin T

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Protein Misfolding Cyclic Amplification of Prions

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells

Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis

Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens

Fecal (micro) RNA Isolation

Tools for the Real-Time Assessment of a Pseudomonas aeruginosa Infection Model

Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells

Read Article