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超薄 Porated 弹性水凝胶作为双细胞培养的仿生基底膜
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JoVE 신문 생체공학
Ultrathin Porated Elastic Hydrogels As a Biomimetic Basement Membrane for Dual Cell Culture
DOI:

11:34 min

December 26, 2017

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Chapters

  • 00:05Title
  • 00:58Synthesis of Zinc Oxide Needles and Addition of Sacrificial Zinc Layer on Microscope Slides
  • 02:55Preparation of Polyethylene Glycol (PEG) Solution and Synthesis of PEG Gel
  • 04:43Release of PEG Gels from Glass Slides
  • 06:12Seeding Cell Bilayers
  • 08:17Immunofluorescence
  • 09:40Results: Immunofluorescence Images of Hydrogels Supporting Monolayer and Bilayer Culture
  • 10:28Conclusion

Summary

자동 번역

目前的双层培养模式不允许功能性的体外研究, 模仿体内环境。使用聚乙二醇和氧化锌模板化方法, 该协议描述了一个超薄仿生基底膜的发展, 具有可调谐的刚度, 孔隙率和生物化学组成, 密切模仿在体内细胞外基质。

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