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JoVE Journal Genetics
在非活动 X 染色体上重新激活 MeCP2 的池 shRNA 屏幕
 
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在非活动 X 染色体上重新激活 MeCP2 的池 shRNA 屏幕

Article DOI: 10.3791/56398-v • 11:15 min • March 2nd, 2018
March 2nd, 2018 •
1,2, 1, 1, 1, 1, 1, 1, 3, 1,4
1Clinical Research Division, Fred Hutchinson Cancer Research Center, 2Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 3Epigenetics Program, Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 4Departments of Medicine and Biochemistry, University of Washington

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我们报告一个小发夹 RNA (shRNA) 和下一代测序协议, 以确定在小鼠细胞系的 X 染色体失活的监管者与萤火虫荧光素酶和潮霉素抵抗基因融合到甲基 CpG 结合蛋白 2 (MeCP2)非活性 X 染色体上的基因。

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遗传学 问题 133 X 染色体失活 表观遗传学 MeCP2 Rett 综合征 XIST shRNA 筛查
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