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Chapters
- 00:05Title
- 00:56Growth and Collection of Vegetative D. discoideum
- 02:51Acute Mechanical Stimulation Using an Orbital Shaker
- 04:18Acute Mechanical Stimulation Using a Microfluidic Chamber
- 06:33Applying Pharmacological Treatments Using the Microfluidic Chamber
- 07:48Results: Observed Shear Force Responses in D. discoideum
- 09:18Conclusion
Here we describe methods for assessing cellular response to acute mechanical stimulation. In the microscopy-based assay, we examine localization of fluorescently-labeled biosensors following brief stimulation with shear flow. We also test activation of various proteins of interest in response to acute mechanical stimulation biochemically.
