Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for <em>In Vitro</em> and <em>In Vivo</em> Applications

Matthias Becker; Janita A. Maring; Barbara Oberwallner; Benjamin Kappler; Oliver Klein; Volkmar Falk; Christof Stamm

doi:10.3791/56419

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Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

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Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

DOI:

10.3791/56419-v

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08:41 min

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December 04, 2017

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Matthias Becker², Janita A. Maring², Barbara Oberwallner, Benjamin Kappler, Oliver Klein², Volkmar Falk^3,4,5, Christof Stamm^3,4

¹Charité – Universitätsmedizin Berlin,corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, ²Berlin-Brandenburg Center for Regenerative Therapies (BCRT), ³German Center for Cardiovascular Research (DZHK), ⁴Deutsches Herzzentrum Berlin (DHZB), ⁵Department of Cardiovascular Surgery, Charité – Universitätsmedizin Berlin,corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health

Chapters

00:05Title
00:43Sectioning Human Myocardium into Slices
02:27Decellularization of Human Myocardium Slices
04:20Processing the Decellularized cECM to a Powder
05:50Enzyme-based Homogenization of cECM Micro-particles
06:56Results: Effective Removal of Cellular Material Enhances Analysis
08:10Conclusion

Summary

자동 번역

This protocol describes the complete decellularization of human myocardium while preserving its extracellular matrix components. Further processing of the extracellular matrix results in the production of microparticles and a cytoprotective self-assembling hydrogel.

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

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