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Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity
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Chapters
- 00:05Title
- 00:49Plate-based Binding Assay for Evaluating Loss of Higher Order Norovirus Capsid Structure
- 04:44Dynamic Light Scattering (DLS) Detecting Virus Aggregation After Heat Treatment
- 06:32DLS for Detecting Virus Aggregation in Real Time
- 07:29Transmission Electron Microscopy (TEM) Sample Preparation
- 09:34Results: Reduction of Human Norovirus Capsid Stability After Heating
- 11:36Conclusion
Routine detection methods utilizing viral genome amplification are limited by their inability to discriminate infectious from non-infectious particles. The purpose of this article is to provide detailed protocols for alternative methods to aid in discrimination of infectious norovirus particles using aptamer binding, dynamic light scattering, and transmission electron microscopy.
