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Chapters
- 00:05Title
- 00:32NIH-3T3 Cell Preparation
- 03:30SPEED Microscopy
- 05:48Results: 3D Spatial Location of the Transport Route for SSTR3 in Ciliary Transition Zone and IFT20 Inside the Cilia Shaft
- 07:18Conclusion
Recently we mapped the three-dimensional (3D) spatial locations of transport routes for various proteins translocating inside primary cilia in live cells. Here this paper details the experimental setup, the process of biological samples and the data analyses for the 3D super-resolution fluorescence imaging approach newly applied in live primary cilia.
