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高分辨率光学制图对小鼠心房电生理的评价
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JoVE 신문 의학
Electrophysiological Assessment of Murine Atria with High-Resolution Optical Mapping
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高分辨率光学制图对小鼠心房电生理的评价

DOI:
10.3791/56478-v

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08:19 min

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February 22, 2018

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, , , , ,
1Department of Bio-informational Pharmacology, Medical Research Institute,Tokyo Medical and Dental University, 2Department of Biofunctional Informatics,Tokyo Medical and Dental University

Chapters

  • 00:04Title
  • 00:48Mouse Cannulation and Perfusion for Optical Mapping using Langendorff
  • 03:37Staining for Single Recording Membrane Voltage, Burst Pacing, and Extra-stimulus Pacing
  • 06:37Results: Conduction Patterns during Normal Sinus Rhythm and Atrial Tachyarrhythmia
  • 07:49Conclusion

Summary

자동 번역

자동 번역

该协议描述了利用具有高时间和空间分辨率的光学映射系统对小鼠心房的电生理评价, 包括膜电压的双记录和 Ca2 +瞬态的编程通过专门的电极导管进行刺激。

Tags

Electrophysiological AssessmentMurine AtriaHigh-resolution Optical MappingCardiac ElectrophysiologyAtrial ArrhythmiaLangendorff ApparatusPerfusion CircuitAnesthesiaHeart ExcisionTyrode’s SolutionPerfusion PressureVena CavaGlass ChamberVentricular CavityStimulation ElectrodeElectrocardiogram

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