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Chapters
- 00:05Title
- 00:56Collection of Placental Tissue
- 01:42Isolation of Trophoblasts from Vilous Tissue
- 05:11Density Centrifugation
- 06:29Plating Cells
- 07:18Treating Cells with TNFα and Harvesting Cells and Total Protein
- 09:10Results: TNFα Regulates the Expression of Rubicon in Trophoblasts from Lean Pregnancies with Female Fetuses
- 10:20Conclusion
Presented here is a protocol for sampling of human placental villous tissue followed by isolation of cytotrophoblasts for primary cell culture. Treatment of trophoblasts with TNFα recapitulates inflammation in the obese intrauterine environment and facilitates the discovery of molecular targets regulated by inflammation in placentas with maternal obesity.
