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从果蝇幼虫神经肌肉交界处的突触电流的焦 Macropatch 记录

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Focal Macropatch Recordings of Synaptic Currents from the Drosophila Larval Neuromuscular Junction

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从果蝇幼虫神经肌肉交界处的突触电流的焦 Macropatch 记录

DOI:

10.3791/56493-v

•

07:01 min

•

September 25, 2017

•

Alexander Vasin, Maria Bykhovskaia²

¹Department of Neurology, School of Medicine,Wayne State University, ²Department of Anatomy and Cell Biology, School of Medicine,Wayne State University

Chapters

00:05Title
00:39Fabrication of Recording Electrodes
01:53Additional Preparation
03:17EJC Electrical Recording
04:33EJC Electrical Stimulation and Seal Resistance
05:34Results: Accurate Detection of Release Events
06:29Conclusion

Summary

자동 번역

在果蝇第三龄幼虫神经肌肉交界处, 可从可视化突触 boutons 局部记录突触电流。这种技术可以监测单个突触溥的活动。

Tags

Keywords: Drosophila Larval Neuromuscular Junction Synaptic Currents Focal Macropatch Recordings Synaptic Boutons Synaptic Transmission Synaptic Proteins Microelectrode Hemolymph-like Solution HL3 Solution Stimulation Pipettes Dissection Plates Wandering Third Instar Larvae Fileting Pinning Nerve Cutting

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