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Fractionnement subcellulaire pour l’Activation de ERK par traitement de peptides dérivés mitochondriale
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JoVE 신문 생물학
Subcellular Fractionation for ERK Activation Upon Mitochondrial-derived Peptide Treatment
DOI:

07:55 min

September 25, 2017

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Chapters

  • 00:05Title
  • 00:52Peptide Treatment to Cells
  • 01:46Subcellular Fractionation of Cytosol and Nucleus
  • 03:31Isolate Crude Mitochondrial Fraction
  • 05:43Results: Analysis of ERK Activation Upon Mitochondrial-derived Peptide Treatment
  • 07:17Conclusion

Summary

자동 번역

Ce protocole décrit comment stimuler les cellules avec des peptides dérivés mitochondriale et évaluer la signalisation cascade et la localisation des phospho-protéines.

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