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अलगाव साल्मोनेला typhimurium-मैक्रोफेज से युक्त Phagosomes का
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages
DOI:

10:33 min

October 25, 2017

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Chapters

  • 00:05Title
  • 01:03Salmonella typhimurium Culture
  • 02:17Coating of Bacteria with NHS-biotin/Streptavidin-conjugated Magnetic Beads
  • 04:38Bone Marrow-derived Macrophages (BMDM) Infection
  • 06:18Isolation of S. typhimurium-containing Phagosomes
  • 08:21Results: Successful Biotinylation of Bacterial and Isolation of Bacterial-containing Phagosomes
  • 09:41Conclusion

Summary

자동 번역

हम यहां के अलगाव के लिए एक सरल और त्वरित विधि का वर्णन साल्मोनेला typhimurium-मैक्रोफेज से युक्त phagosomes द्वारा बायोटिन और streptavidin के साथ बैक्टीरिया कोटिंग ।

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