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连续切片电子断层扫描显示的 3D果蝇间接飞行肌的线粒体超微结构研究

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3D Mitochondrial Ultrastructure of Drosophila Indirect Flight Muscle Revealed by Serial-section Electron Tomography

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连续切片电子断层扫描显示的 3D果蝇间接飞行肌的线粒体超微结构研究

DOI:

10.3791/56567-v

•

06:45 min

•

December 19, 2017

•

Yi-fan Jiang, Hsiang-ling Lin, Chi-yu Fu

¹Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine,National Taiwan University, ²Institute of Cellular and Organismic Biology,Academia Sinica

Chapters

00:05Title
00:37Sectioning Drosophila Tissues with Vibrating Blade Microtome
01:19High-pressure Freezing (HPF) and Freeze Substitution (FS) for Electron Microscope (EM)
02:30Serial-sectioning for Electron Tomography
03:42Double-title Electron Tomography
05:09Results: Integrated Intra-mitochondria Cristae and Matrix Patterns in 3D
06:20Conclusion

Summary

자동 번역

在本协议中, 我们展示了在果蝇间接飞行肌肉中应用的序列切片电子断层扫描来阐明线粒体结构。

Tags

Keywords: 3D Mitochondrial Ultrastructure Drosophila Indirect Flight Muscle Serial-section Electron Tomography Cellular Ultrastructure High-pressure Freezing Free Substitution Resin Embedding Ultramicrotomy Transmission Electron Microscope Dual-axis Tomography

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