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Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol
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JoVE 신문 암 연구학
Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol
DOI:

06:14 min

March 12, 2018

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Chapters

  • 00:05Title
  • 01:11Culture Plate, Collagen Gel Preparation, and Cell Imaging
  • 03:33Results: Representative Inverted Vertical Invasion Assay Analyses
  • 04:33Conclusion

Summary

자동 번역

This protocol describes an inverted vertical invasion assay that could be used to quantify the migration and invasion capabilities of cells in a three-dimensional setting while preserving the cell microenvironment. This assay is suitable for rare and/or sensitive cells.

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