Journal
/
생물학
/
Standardized Method for High-throughput Sterilization of Arabidopsis Seeds
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

DOI:
10.3791/56587-v

•

08:13 min

•

October 17, 2017

•

, , , ,
1Arabidopsis Biological Resource Center, Center for Applied Plant Sciences,The Ohio State University, 2Department of Molecular Genetics, Center for Applied Plant Sciences,The Ohio State University

Chapters

  • 00:05Title
  • 00:41Sterilization of Arabidopsis Seeds with Bleach
  • 02:14Sterilization of Arabidopsis Seed with Chlorine Gas
  • 05:01Seed Plating and Evaluating Germination
  • 05:59Results: Effects of Bleach or Chlorine Gas Sterilization on Seed Germination
  • 07:28Conclusion

Summary

자동 번역

자동 번역

The objective of this study was to determine the effects of bleach and chlorine gas sterilization on seed germination of a range of Arabidopsis genotypes grown on sterile media. Optimized sterilization protocols have been developed to prevent the growth of microbial contaminants while providing satisfactory seed survival.

Tags

Keywords: Arabidopsis SeedsSeed SterilizationHigh-throughputBleach SolutionTWEEN 20Laminar Flow HoodChlorine GasHydrochloric AcidGermination RateGenomicsProteomicsMetabolomics

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/56587?article_type=v

A high-throughput method to globally study the organelle morphology in S. cerevisiae
check_url/kr/56587?article_type=v

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
check_url/kr/56587?article_type=v

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
check_url/kr/56587?article_type=v

High-throughput Saccharification Assay for Lignocellulosic Materials
check_url/kr/56587?article_type=v

Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping
check_url/kr/56587?article_type=v

High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method
check_url/kr/56587?article_type=v

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice
check_url/kr/56587?article_type=v

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds
check_url/kr/56587?article_type=v

Recovery of Adult Zebrafish Hearts for High-throughput Applications
check_url/kr/56587?article_type=v

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Read Article