JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 00:46Heterologous Expression of His-tagged RTA in E. coli
- 02:12Purification of His-tagged RTA via Affinity Chromatography
- 04:21Yeast Transformation and Cell Wall Removal
- 05:28GFP Reporter Assay Measurement in 96-well Plates
- 06:57Results: Assay to Identify Cellular Components Required for RTA Trafficking in Yeast
- 08:30Conclusion
Tags
Keywords:
Fluorescence-based Reporter AssayRicin Toxin A Chain (RTA)YeastCellular ComponentsTraffickingIntercellular TransportRibosomeCytotoxic SubunitPlasma MembraneCytosomeCell TransformationLB Kanamycin MediumOptical DensityIPTGCentrifugationBinding BufferSonicationAffinity PurificationNickel-based ColumnHIS-tagged RTA
