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单分子荧光原位杂交实验中单个大肠埃希菌细胞的转录标记方法
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Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments
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单分子荧光原位杂交实验中单个大肠埃希菌细胞的转录标记方法

DOI:
10.3791/56600-v

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07:51 min

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December 21, 2017

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, ,
1Department of Physics of Complex Systems,Weizmann Institute of Science

Chapters

  • 00:05Title
  • 00:42Sample Fixation
  • 02:22Sample Permeabilization
  • 03:10Hybridization
  • 04:26Washing
  • 05:03Sample Preparation for Imaging
  • 06:12Results: Representative Results of Two Different Transcripts Labeled by the smFISH Method
  • 07:25Conclusion

Summary

자동 번역

자동 번역

这篇手稿描述了一种方法, 标记个别信使 RNA (mRNA) 转录与荧光标记的 DNA 探针, 用于分子荧光原位杂交 (smFISH) 实验在大肠杆菌. smFISH 是一种可视化方法, 它允许在固定的单个细胞中同时检测、定位和量化单个 mRNA 分子。

Tags

Escherichia ColiSingle-molecule Fluorescence In Situ Hybridization (smFISH)TranscriptsCell-to-cell VariabilityCell Wall LocalizationLB MediumOptical DensityPBSFixation BufferPermeabilizationEthanolWash BufferOligonucleotide Probe SetHybridization Buffer

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