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Aislamiento y cultivo de progenitores neuronales seguido por inmunoprecipitación de cromatina de histona 3 lisina 79 dimetilación marca
JoVE 신문
신경과학
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JoVE 신문 신경과학
Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
DOI:

10:09 min

January 26, 2018

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Chapters

  • 00:05Title
  • 00:49Isolation of Cerebellar Granular Neuron Progenitors (CGNP)
  • 03:59Cell Fixation and Chromatin Shearing
  • 05:53Preclearing
  • 06:47Preparation of Antibody-Coupled Beads
  • 07:37Chromatin Immunoprecipitation
  • 08:51Results: H3K79me2 ChIP-qPCR and ChIP-Seq Analysis
  • 09:41Conclusion

Summary

자동 번역

Presentamos un método eficaz y reproducible para aislar las células progenitoras neurales de tejido cerebral embrionario y postnatal de inmunoprecipitación de cromatina (ChIP) de la histona 3 lisina 79 dimetilación (H3K79me2) - una marca de histona situada dentro de la cultura el dominio globular de la histona 3.

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