JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Department of Physiology, School of Basic Medical Sciences,Southern Medical University, 2School of Basic Medical Sciences,Guizhou Medical University, 3Department of Laboratory Medicines, Guangzhou General Hospital of Guangzhou Military Region,Southern Medical University, 4Experiment Teaching Center, School of Basic Medical Sciences,Southern Medical University
Chapters
- 00:04Title
- 00:56Auditory Brainstem Response Recording
- 02:12The Organ of Corti Dissection
- 03:22Immunofluorescence Staining
- 05:21Patch Clamp Recording of Isolated OHCs
- 07:33Results: Whole-Cell Patch Clamp Recording of Isolated Outer Hair Cells
- 08:19Conclusion
Ce protocole décrit les méthodes pour enregistrer la réponse auditif du tronc cérébral de ratons postnatal. Pour examiner le développement fonctionnel des cellules ciliées externes, la procédure expérimentale de cellules entières patch clamp d’enregistrement en isolé les cellules ciliées externes est décrit étape par étape.
