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Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers
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1Division of Pathology,Fondazione IRCCS Ca’ Granda, Ospedale Maggiore Policlinico, 2School of Medicine,University of Milan, 3School of Biology,University of Pavia, 4Division of Breast Surgery,Fondazione IRCCS Ca’ Granda – Ospedale Maggiore Policlinico, 5Division of Medical Oncology,Fondazione IRCCS Ca’ Granda – Ospedale Maggiore Policlinico, 6Department of Biomedical, Surgical, and Dental Sciences,University of Milan
Chapters
- 00:05Title
- 01:24Design and Construct Tissue Microarrays (TMAs) Based on the Kononen Technique
- 06:20Silver In Situ Hybridization (SISH) Analysis of Human Epidermal Growth Factor Receptor 2 (HER2)
- 09:11Results: Analysis of Heterogeneity of HER2 Gene Amplification in Breast Cancers
- 10:00Conclusion
Heterogeneous distribution of HER2-positive cells can be observed in a subset of breast cancers and generates clinical dilemmas. Here, we introduce a reliable and cost-effective protocol to define, quantify, and compare HER2 intra-tumor genetic heterogeneity in a large series of heterogeneously processed breast cancers.
