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1Centre for Infectious Diseases and Microbiology-Public Health,Westmead Hospital, 2Centre for Infectious Diseases and Microbiology Laboratory Services,ICPMR, 3Marie Bashir Institute for Infectious Diseases and Biosecurity,The University of Sydney, 4Department of Microbiology and Infectious Diseases, Canberra Hospital and Health Services,Australian National University Medical School, 5Infection Management Services,Australian National University Medical School, 6Department of Microbiology and Infectious Diseases,St. Vincent’s Hospital, 7Department of Infectious Diseases,Peter MacCallum Cancer Centre
Chapters
- 00:05Title
- 01:11Tagmentation and PCR Amplification
- 03:35PCR Cleanup
- 05:15Library Normalization
- 07:19Results: Susceptibility Profiles of Candida glabrata Isolates Correlates with Presence or Absence of Specific Gene Mutations
- 08:11Conclusion
Este estudo implementado sequenciamento do genoma inteiro para análise de mutações em genes que conferem resistência a drogas antifúngicas no Candida glabrata. Isolados de c. glabrata resistentes à echinocandins, azóis e 5-flucitosina, foram sequenciados para ilustrar a metodologia. Perfis de susceptibilidade dos isolados correlacionaram com a presença ou ausência de padrões específicos de mutação nos genes.
