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从前期到中期二期小鼠卵母细胞进展的染色质传播制剂分析

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Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II

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从前期到中期二期小鼠卵母细胞进展的染色质传播制剂分析

DOI:

10.3791/56736-v

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10:39 min

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February 26, 2018

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Grace H. Hwang*¹, Jessica L. Hopkins*¹, Philip W. Jordan

¹Department of Biochemistry and Molecular Biology,Johns Hopkins University Bloomberg School of Public Health

Chapters

00:04Title
00:59Harvesting Fetal or Neonatal Ovaries
03:55Metaphase I (MI) or Metaphase II (MII) Oocyte Collection
05:17Oocyte Denuding and Zona Pellucida Removal
07:05MI and MII Oocyte Chromatin Spreads
08:08Results: Crossover Formation in C57B/6J Oocytes
09:52Conclusion

Summary

자동 번역

哺乳动物中的卵子发生过程已知易出错, 特别是由于染色体 missegregation。本手稿描述了小鼠前期、中期 I 和 II. 期卵母细胞的染色质传播制备方法。这些基本技术允许研究整个哺乳动物卵子发生过程的染色质束缚蛋白和染色体形态学。

Tags

Chromatin Spread Mouse Oocyte Prophase Metaphase II Oogenesis Chromosome Immunolabeling Ploidy Aneuploidy Infertility Ovary Hypo-extraction Buffer Sucrose

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