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1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital,Central South University, 2Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital,Central South University, 3State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital,Central South University, 4The State Key Laboratory of Medical Genetics,Central South University
Chapters
- 00:04Title
- 01:46Two-dimensional Gel Electrophoresis: IEF (First Dimension)
- 03:12Two-dimensional Gel Electrophoresis: SDS-PAGE (Second Dimension)
- 07:19Staining of 2DE-separated Proteins
- 08:52Results: Separation and Identification of NFPA Tissue Proteome
- 10:33Conclusion
Her præsenterer vi en to-dimensionel gelelektroforese (2DE) kombineret med massespektrometri (MS) til at adskille og identificere menneskelige hypofyseadenom væv proteomet, som præsenterer en god og reproducerbare 2DE mønster. Mange proteiner er observeret i hver 2DE plet når analysere komplekse kræft proteomet med brug af høj-følsomhed MS.
