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1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital,Central South University, 2Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital,Central South University, 3State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital,Central South University, 4The State Key Laboratory of Medical Genetics,Central South University
Chapters
- 00:04Title
- 01:46Two-dimensional Gel Electrophoresis: IEF (First Dimension)
- 03:12Two-dimensional Gel Electrophoresis: SDS-PAGE (Second Dimension)
- 07:19Staining of 2DE-separated Proteins
- 08:52Results: Separation and Identification of NFPA Tissue Proteome
- 10:33Conclusion
Nous présentons ici une électrophorèse bidimensionnelle (2DE) couplée à la spectrométrie de masse (MS) pour séparer et identifier le protéome de tissus Adénome hypophysaire humaine, qui présente un motif de 2DE bon et reproductible. Plusieurs protéines sont observées dans chaque endroit de 2DE lors de l’analyse complexe cancer proteome avec l’utilisation de MS de haute sensibilité.
