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1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital,Central South University, 2Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital,Central South University, 3State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital,Central South University, 4The State Key Laboratory of Medical Genetics,Central South University
Chapters
- 00:04Title
- 01:46Two-dimensional Gel Electrophoresis: IEF (First Dimension)
- 03:12Two-dimensional Gel Electrophoresis: SDS-PAGE (Second Dimension)
- 07:19Staining of 2DE-separated Proteins
- 08:52Results: Separation and Identification of NFPA Tissue Proteome
- 10:33Conclusion
Hier präsentieren wir Ihnen eine zweidimensionale Gelelektrophorese (2DE) gekoppelt mit Massenspektrometrie (MS) zu trennen und menschlichen Hypophysen Adenom Gewebe Proteom, stellt eine gute und reproduzierbare 2DE Muster identifizieren. Viele Proteine werden in jedem 2DE Fleck beobachtet, bei der Analyse von komplexen Krebs Proteome mit dem Einsatz von hochempfindlichen MS.
