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1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital,Central South University, 2Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital,Central South University, 3State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital,Central South University, 4The State Key Laboratory of Medical Genetics,Central South University
Chapters
- 00:04Title
- 01:46Two-dimensional Gel Electrophoresis: IEF (First Dimension)
- 03:12Two-dimensional Gel Electrophoresis: SDS-PAGE (Second Dimension)
- 07:19Staining of 2DE-separated Proteins
- 08:52Results: Separation and Identification of NFPA Tissue Proteome
- 10:33Conclusion
Aquí, presentamos una electroforesis en gel bidimensional (2DE) acoplada con espectrometría de masas (MS) para separar e identificar adenoma pituitario humano tejido proteoma, que presenta un patrón de 2DE buena y reproducible. Muchas proteínas se observan en cada punto de 2DE al analizar el proteoma del complejo del cáncer con el uso de MS de alta sensibilidad.
