An Efficient <em>In Vitro</em> Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector

Daniela Benati; Fabienne Cocchiarella; Alessandra Recchia

doi:10.3791/56742

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생물학

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一种有效的体外转位方法由转录调节的睡眠美容系统封装到一个集成缺陷的慢向量

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An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector

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一种有效的体外转位方法由转录调节的睡眠美容系统封装到一个集成缺陷的慢向量

DOI:

10.3791/56742-v

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10:13 min

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January 12, 2018

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Daniela Benati, Fabienne Cocchiarella, Alessandra Recchia

¹Centre for Regenerative Medicine, Department of Life Sciences,University of Modena and Reggio Emilia

Chapters

00:05Title
00:51Transduction of Human Primary Keratinocytes
04:31Cytofluorimetric Analysis of Transduced Primary Keratinocytes
06:24Quantitative RT-PCR (qRT-PCR)
07:24Results: Sleeping Beauty System Achieves Stable Integration of a GOI in the Human Genome
08:40Conclusion

Summary

자동 번역

该协议描述了一种方法, 以实现稳定的基因的兴趣进入人类基因组由转录调控的睡美人系统。本文报道了有缺陷的慢载体的合成、人体细胞的体外转导和转细胞的分子检测。

一种有效的体外转位方法由转录调节的睡眠美容系统封装到一个集成缺陷的慢向量

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Summary

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