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Kombinere kemisk Cross-linking og massespektrometri af intakt proteinkomplekser at studere arkitektur af multi subunit Protein forsamlinger
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JoVE 신문 생물학
Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Kombinere kemisk Cross-linking og massespektrometri af intakt proteinkomplekser at studere arkitektur af multi subunit Protein forsamlinger

DOI:
10.3791/56747-v

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10:01 min

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November 28, 2017

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, , , , ,
1Interdisciplinary research center HALOmem,Martin Luther University Halle-Wittenberg, 2Department of Chemistry,Kings College London

Chapters

  • 00:05Title
  • 00:51Mass Spectrometry-based Protein Identification
  • 04:35Mass Spectrometry of Intact Protein Complexes (Native Mass Spectrometry)
  • 06:17Chemical Cross-linking Coupled with Mass Spectrometry
  • 07:19Results: Chemical Cross-linking and Mass Spectrometry of Multi-subunit Protein Complexes
  • 09:14Conclusion

Summary

자동 번역

자동 번역

Arkitektur af proteinkomplekser er afgørende for deres funktion. Kombinere forskellige massespektrometriske metoder vist sig magtfulde at studere deres forsamling. Vi leverer protokoller for kemiske cross-linking og indfødte massespektrometri og viser hvordan disse supplerende teknikker bidrage til at belyse arkitektur af multi subunit protein forsamlinger.

Tags

Chemical Cross-linkingMass SpectrometryIntact Protein ComplexesMulti-subunit Protein AssembliesStructural InformationCompositionConnectivityStoichiometryProtein Complex PurificationElectrophoresisCoomassie StainingProtein Band ExcisionDisulfide ReductionCysteine AlkylationTrypsin DigestionPeptide Extraction

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