/

면역학 및 감염병학

/

一种新型大鼠自然杀伤细胞无饲养系统的研究体外

JoVE 신문
면역학 및 감염병학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 면역학 및 감염병학

A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro

Please note that all translations are automatically generated. Click here for the English version.

一种新型大鼠自然杀伤细胞无饲养系统的研究体外

DOI:

10.3791/56785-v

•

10:36 min

•

January 09, 2018

•

Patrick Ming-Kuen Tang², Philip Chiu-Tsun Tang, Jeff Yat-Fai Chung, Jessica Shuk Chun Hung, Qing-Ming Wang, Guang-Yu Lian, Jingyi Sheng, Xiao-Ru Huang, Ka-Fai To, Hui-Yao Lan

¹Department of Anatomical and Cellular Pathology,The Chinese University of Hong Kong, ²Li Ka Shing Institute of Health Sciences and Department of Medicine & Therapeutics,The Chinese University of Hong Kong

Chapters

00:05Title
01:24Preparation of OP9 Conditional Medium
02:55Isolation of Mouse Bone Marrow Cells
06:14siRNA-mediated Gene Silencing of Differentiating Natural Killer Cells
06:43Analysis of Natural Killer Cell Differentiation Using Flow Cytometry
07:35Results: Study of siRNA Dependent Gene Silencing on the Differentiation of Bone Marrow Derived Natural Killer Cells in a Feeder-free In Vitro System
09:27Conclusion

Summary

자동 번역

在这里, 我们提出了一个 mass-produce 基因沉默小鼠 NK 细胞的协议, 使用无馈线分化系统的机械研究体外和在体内。

Tags

Keywords: Feeder-free System Mass Production Murine Natural Killer Cells In Vitro Innate Immunology Natural Killer Cell Development Cancer Immunity Bone Marrow Personal Immunotherapy OP9 Cells Alpha-MEM Medium Phosphate-buffered Saline Pentobarbitone Femur Bones

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

制备和使用艾滋病毒感染主的CD4 + T细胞作为靶细胞，自然杀伤细胞的细胞毒性检测

人工抗原提呈细胞（AAPC）的自然杀伤T细胞介导的激活和扩展

杀手人工抗原提呈细胞（KaAPC）为有效<em>在体外</em>人抗原特异性T细胞耗竭

一种新型<em>在体外</em>模型研究人类全血细胞间的相互作用

的自然杀伤细胞免疫突触上的玻璃支承平面脂双层超分辨率成像

在体外产生的常见淋巴祖使用AC-6馈线系统的小鼠浆细胞样树突细胞

从小鼠脾隔离和扩大不变的自然杀伤T细胞的优化方法

人全血中NK细胞杀伤活性的流式细胞仪分析

诱导麻痹及视觉系统损伤小鼠 T 细胞特异的视神经脊髓炎抗原水通道蛋白-4

一种新的体外创面愈合试验评价细胞迁移

Read Article