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En roman Feeder-fri ordning for masseproduktion af Murine naturlige dræberceller In Vitro
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro
DOI:

10:36 min

January 09, 2018

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Chapters

  • 00:05Title
  • 01:24Preparation of OP9 Conditional Medium
  • 02:55Isolation of Mouse Bone Marrow Cells
  • 06:14siRNA-mediated Gene Silencing of Differentiating Natural Killer Cells
  • 06:43Analysis of Natural Killer Cell Differentiation Using Flow Cytometry
  • 07:35Results: Study of siRNA Dependent Gene Silencing on the Differentiation of Bone Marrow Derived Natural Killer Cells in a Feeder-free In Vitro System
  • 09:27Conclusion

Summary

자동 번역

Vi præsenterer her, en protokol til at masseproducere genhæmning murine NK celler ved hjælp af en feeder-fri differentiering system for mekanistisk undersøgelse in vitro og in vivo.

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