Microscopy Based Methods for the Assessment of Epithelial Cell Migration During  <em>In Vitro</em> Wound Healing

00:05Title
01:07Artificial Wound Scratch Assay
03:19Artificial Wound Image Analysis and Migration Quantification
04:48Artificial Migration Front Assay
07:07Results: Representative Artificial Wound Scratch and Migration Front Assay Imaging
08:00Conclusion

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This manuscript describes how incision-like lesions made on cultured epithelial cell monolayers conveniently model wound healing in vitro, allowing for imaging by confocal or laser scanning microscopy, and which can provide high-quality quantitative and qualitative data for studying both cell behavior and the mechanisms involved in migration.

Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing

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