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报告酶荧光法检测小鼠结核分枝杆菌的影像学
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence
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报告酶荧光法检测小鼠结核分枝杆菌的影像学

DOI:
10.3791/56801-v

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10:06 min

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February 26, 2018

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, , ,
1Department of Microbial Pathogenesis and Immunology,Texas A&M Health Science Center, 2Tuberculosis Research Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases,National Institutes of Health

Chapters

  • 00:04Title
  • 01:02Preparing the M. tuberculosis Inoculum
  • 03:13Inoculating Mice with Airborne M. tuberculosis
  • 05:33Reporter Enzyme Fluorescence Imaging and Analysis of Lung Homogenate
  • 08:05Results: Imaging
  • 09:32Conclusion

Summary

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자동 번역

我们描述了使用报告酶荧光 (REF) 感染结核分枝杆菌(m 结核) 的小鼠的光学成像。该协议有助于在临床前动物模型中对m. 结核进行敏感和特定的检测, 以进行发病机制、治疗和疫苗研究。

Tags

Keywords: Reporter Enzyme FluorescenceMycobacterium TuberculosisPre-clinical Animal ModelsPathogenesisTherapeuticsVaccine ResearchVirulence MechanismsHost-pathogen InteractionsMOADCTW Medium7H11 PlatesCFUBiosafety HoodNebulizerInoculation Chamber

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