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1Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, 2College of Life Sciences,University of Chinese Academy of Sciences, 3School of Life Science and Technology,Shanghai Tech University, 4Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences
Chapters
- 00:04Title
- 00:54sgRNA Surveyor Nuclease Assay
- 02:50Cas9 mRNA Production
- 04:11sgRNA Production
- 05:30Embryo Collection, Microinjection, and In Vitro Culture
- 06:43Results: Superior Integration with Cas9
- 07:46Conclusion
Die gruppierten regelmäßig dazwischen kurze palindromische Wiederholungen/CRISPR verbundenen Protein 9 (CRISPR/Cas9) System bietet ein viel versprechendes Instrument für Gentechnik und eröffnet die Möglichkeit, gezielte Integration der transgene. Wir beschreiben eine Homologie-vermittelte Ende verbinden (HMEJ)-basierte Strategie für effiziente DNA Integration in Vivo gezielte und gezielte Gentherapien verwenden CRISPR/Cas9.
