Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / 用生物层干涉法捕获小分子离子通道蛋白的相互作用动力学…
JoVE Journal
Biochemistry

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE Journal Biochemistry
用生物层干涉法捕获小分子离子通道蛋白的相互作用动力学
 
Click here for the English version

用生物层干涉法捕获小分子离子通道蛋白的相互作用动力学

Article DOI: 10.3791/56846-v • 10:41 min • March 7th, 2018
March 7th, 2018 •
1, 2, 1, 3
1Department of General Surgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 2Key Laboratory of Systems Biomedicine (Ministry of Education), Institute of Systems Biomedicine, Shanghai Jiao Tong University, 3Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine

챕터

요약
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

该协议描述了纯化 hEAG1 离子通道蛋白与小分子脂配体磷酸肌醇4、5-bisphosphate (PIP2) 的相互作用。实验表明, BLI 可能是一种新的小分子离子通道配体筛选的潜在方法。

Tags

生物化学 问题 133 生物层干涉 (BLI) 离子通道蛋白 小分子 蛋白质亲和纯化 哺乳动物稳定表达系统 人 EAG1 (hEAG1) 通道 药物发现 治疗靶点 配体筛选
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter