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用于化学诱导的 DNA 化和表观遗传重塑的基因工程 Split-TET2 酶
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An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling
DOI:

08:34 min

December 18, 2017

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Chapters

  • 00:05Title
  • 00:48Cell Culture, Plasmid Transfection, and Chemical Induction
  • 01:45Quantification of Rapamycin-indiced 5-hydroxymethylcystosine (5hmc) Production by Immunostaining
  • 03:08Dot-blot Assay Quantify 5hmc and 5-methylcystosine (5mc) Amounts
  • 07:03Results: Validation of Chemically Induced 5hmc Levels
  • 07:58Conclusion

Summary

자동 번역

介绍了将工程化的 split-TET2 酶 (苹果酒) 引入哺乳动物细胞中用于化学诱导 DNA 化和后生重塑的详细协议。

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