This content is Free Access.
1Department of Pathology, Immunology and Experimental Medicine,University of Florida, 2Heller School for Social Policy and Management,Brandeis University, 3Department of Medicine, College of Medicine,University of Florida, 4Department of Biomedical Engineering, College of Engineering,University of Florida, 5Department of Pediatrics, College of Medicine,University of Florida
Chapters
- 00:04Title
- 00:35Pancreas Fixation and Hydrogel Embedding
- 03:37Tissue Clearing
- 04:38Immunofluorescence Staining
- 07:03Mounting Samples for Imaging
- 08:30Results: Human Neuro-insular Network and 3D Schwann Cell Visualization
- 09:35Conclusion
Here, we present a protocol to image human pancreas sections in three dimensions (3D) using optimized passive clearing methods. This manuscript demonstrates these procedures for passive optical clearing followed by multiple immunofluorescence staining to identify key elements of the autonomic and sensory neural networks innervating human islets.
