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1Department of Pathology, Immunology and Experimental Medicine,University of Florida, 2Heller School for Social Policy and Management,Brandeis University, 3Department of Medicine, College of Medicine,University of Florida, 4Department of Biomedical Engineering, College of Engineering,University of Florida, 5Department of Pediatrics, College of Medicine,University of Florida
Chapters
- 00:04Title
- 00:35Pancreas Fixation and Hydrogel Embedding
- 03:37Tissue Clearing
- 04:38Immunofluorescence Staining
- 07:03Mounting Samples for Imaging
- 08:30Results: Human Neuro-insular Network and 3D Schwann Cell Visualization
- 09:35Conclusion
Aquí, presentamos un pasivo de las secciones de páncreas humano de imagen en tres dimensiones (3D) mediante protocolo optimizado métodos de compensación. Este manuscrito muestra estos procedimientos para pasivo óptico claro seguido por múltiples inmunofluorescencia que manchaba para identificar los elementos claves de las redes neuronales autonómicas y sensoriales inervan islotes humanos.
