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Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion
DOI:

08:50 min

March 14, 2018

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Chapters

  • 00:05Title
  • 00:47Preparing Acid-cleaned Cover Glass
  • 01:59Preparing of Fusogenic Optical-quality Surfaces
  • 02:52Micropatterning Hydrocarbon-containing Compounds to Confine Fusion to Predetermined Regions
  • 03:58Fabricating Glass Bottom Dishes
  • 04:55Collecting Thioglycollate-elicited Macrophages
  • 06:15Results: Macrophage Fusion on Glass Surfaces
  • 07:51Conclusion

Summary

자동 번역

This protocol describes the fabrication of optical-quality glass surfaces adsorbed with compounds containing long-chain hydrocarbons that can be used to monitor macrophage fusion of living specimens and enables super-resolution microscopy of fixed specimens.

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