Metabolische Etikettier- und Profilierung des Transfer-RNAs mit Macroarrays

Article DOI: 10.3791/56898-v • 10:56 min • January 16th, 2018

January 16th, 2018 •

Sophia Emetu*¹, Morgan Troiano*¹, Jacob Goldmintz*¹, Jensen Tomberlin¹, Simon Grelet², Philip H. Howe², Christopher Korey³, Renaud Geslain¹

¹Laboratory of tRNA Biology, Department of Biology, College of Charleston, ²Department of Biochemistry and Molecular Biology, MUSC, ³Department of Biology, College of Charleston

* These authors contributed equally

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Wir beschreiben eine original Transfer-RNA-Analyse-Plattform namens SPOt (optimierte Plattform für die Beobachtung der tRNA). Ort und Stelle misst simultan zelluläre Ebenen alle tRNAs in biologischen Proben, in nur drei Schritten und in weniger als 24 Stunden.

Metabolische Etikettier- und Profilierung des Transfer-RNAs mit Macroarrays

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