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Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts
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신경과학
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Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts

Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts

DOI:
10.3791/56904-v

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09:07 min

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February 05, 2018

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, , , ,
1Department of Experimental Medical Science, Wallenberg Neuroscience Center, Division of Neurobiology and Lund Stem Cell Center,Lund University, 2John van Geest Centre for Brain Repair & Department of Neurology, Department of Clinical Neurosciences and Cambridge Stem Cell Institute,University of Cambridge

Chapters

  • 00:04Title
  • 01:02Preparation of Skin Fibroblasts for Reprogramming
  • 01:43Plating for Reprogramming (Day 1)
  • 03:22Viral Transduction (Day 0)
  • 04:12Maintenance of the Converting Cells
  • 04:58FACS
  • 07:33Results: Generation of Induced Neurons from Adult Human Dermal Fibroblasts
  • 08:15Conclusion

Summary

자동 번역

자동 번역

Direct neuronal reprogramming generates neurons that maintain the age of the starting somatic cell. Here, we describe a single vector-based method to generate induced neurons from dermal fibroblasts obtained from adult human donors.

Tags

Keywords: Induced NeuronsHuman Adult Skin FibroblastsCell ReprogrammingNeurological DisordersDisease ModelingToxicologyDiagnosticsDrug ScreeningCell CultureFibroblast MediumGelatin CoatingCell SeedingCell CountingCell LysisTrypsinizationCell Suspension

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