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면역학 및 감염병학

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X 射线辐照 Caco-2 细胞与 PBMC 间串扰的共培养方法研究

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC

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X 射线辐照 Caco-2 细胞与 PBMC 间串扰的共培养方法研究

DOI:

10.3791/56908-v

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11:40 min

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January 30, 2018

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Gabriele Babini*¹, Jacopo Morini*¹, Sofia Barbieri, Giorgio Baiocco, Giovanni Battista Ivaldi, Marco Liotta, Paola Tabarelli de Fatis, Andrea Ottolenghi

¹Dipartimento di Fisica,Università degli Studi di Pavia, ²IRCCS Istituti Clinici Scientifici Maugeri

Chapters

00:04Title
01:05Cell Preparation and Irradiation
03:21Cell Metabolic Activity (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide [MTT]) and Viability Assays, and Trans-Epithelial Electrical Resistance (TEER)
06:12Western Blot Analysis
09:15Results: Representative Effects of Caco-2 Cell Radiation Exposure and PBMC Co-culture
11:01Conclusion

Summary

자동 번역

我们提出了一个协议来研究 X 射线辐照 Caco-2 和外周血单个核细胞 (PBMC) 之间的串扰。该协议始于 Caco-2 辐照和与 PBMC 共文化的建立;随后, 反式上皮电阻是定期测量超过48小时和西方印迹执行在 Caco-2 和 PBMC。

Tags

Keywords: Caco-2 Cells PBMCs Coculture Ionizing Radiation Epithelial Cell Permeability Tight Junction Function Radiation Biology Radioimmunotherapy Cell Culture Density Gradient Centrifugation X-ray Irradiation Dosimetry Cell Viability

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